New Amino acids for Protein Engineering
Protein engineering is a powerful tool for design of novel
macromolecular surface arrays, reversibly hydrogels and artificial
extracellular matrices for use in tissue regeneration and
repair. In vivo microbial expressions of artificial genes provide a
means of preparing such non-natural proteins in high yields.
Since the experiments for synthesizing the analogs and tesing them
for incorporation in vivo are tedious we have developed a
fast screening procedure for screening analogs and candidates
for the aminoacyl t-RNA synthetases.
This procedure "HEIR-DOCK" protocol has been successfully applied
to phenyl alanyl synthetase and the analogs.
Phenylalanyl t-RNA synthetase
Predicted Binding site for Phenylalanyl t-RNA synthetase
Analogs Tested for Phenylalanyl t-RNA synthetase
Calculated Binding Energies for the analogs of Phe